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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
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Comparative RNA-seq based transcriptomic analysis of bud dormancy in grape.

Muhammad Khalil-Ur-Rehman1, Long Sun1, Chun-Xia Li1

  • 1Laboratory of Fruit Tree Biotechnology, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

BMC Plant Biology
|January 21, 2017
PubMed
Summary
This summary is machine-generated.

This study reveals key molecular mechanisms of grape bud dormancy, identifying enriched plant hormone pathways like abscisic acid (ABA) and gibberellin (GA). These findings offer insights into regulating dormancy for improved grape cultivation.

Keywords:
DEGsEndodormancyParadormancyRNA-seqSummer buds

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Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Agricultural Science

Background:

  • Bud dormancy is crucial for perennial plant survival in harsh conditions.
  • Grape (Vitis vinifera) is a globally significant fruit crop, yet its bud dormancy mechanisms remain unclear.
  • Understanding grape bud dormancy is vital for agricultural applications.

Purpose of the Study:

  • To elucidate the molecular mechanisms regulating bud dormancy in grape (Vitis vinifera).
  • To identify key genes and pathways involved in different dormancy stages.

Main Methods:

  • Transcriptome and differential transcript expression analysis using Illumina RNA-seq.
  • Analysis of gene expression across three dormancy stages: paradormancy (PD), endodormancy (ED), and summer buds (SB).
  • Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Main Results:

  • Over 6000 differentially expressed transcripts were identified across dormancy stages.
  • KEGG analysis highlighted enriched pathways in secondary metabolites biosynthesis and plant hormone signaling.
  • Abscisic acid (ABA) and gibberellin (GA) pathways were most enriched, with specific transcript patterns observed for PP2C, GID1, and DELLA families.
  • Redox-related differentially expressed transcripts were abundant.

Conclusions:

  • A hypothesis for the molecular mechanism of grape bud dormancy has been proposed.
  • The findings provide a foundation for future research and potential improvements in the grape industry.