Biomass density-function relationships in suspended growth biological processes - A critical review

Good settling performance in suspended growth biomass systems, for example in activated sludge (AS) process, leads to efficient wastewater and sludge treatment. Factors that cause the differences in settleablility of AS include the morphology of bacteria, microbial community structure, and the density of bacteria and flocs. Density of AS at three levels, namely, cell, floc, and process, have been discussed here to explain the variations in AS settleability. Dense materials, inside or outside the cell, significantly increase density of AS bacteria or flocs. Functional bacteria, defined as those performing N and P removal and recovery such as phosphate accumulating organisms, nitrifiers, and anammox contain cellular inclusions that increase their density, and consequently a dense and well-settling biomass results at the process level in those systems. A density based selector of AS can be used to enrich functional bacteria in the process through the wasting and sludge age control operations in AS process. This paper critically reviews the latest literature to elucidate mechanisms of density enhancement from cell to process level, and identifies needs/strategies to improve the AS process through a biomass density selector.