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Draft Genome Sequences of Pandrug-Resistant Serratia marcescens Clinical Isolates Harboring blaNDM-1

Yancheng Yao1, Linda Falgenhauer1, Volkhard A J Kempf2

  • 1Institute of Medical Microbiology, German Center for Infection Research (DZIF Partner Site Giessen-Marburg-Langen), Justus-Liebig University Giessen, Giessen, Germany.

Genome Announcements
|January 21, 2017
PubMed

Insights

Two pandrug-resistant Serratia marcescens strains were sequenced, revealing that antibiotic resistance is plasmid-driven. This highlights a significant reservoir of transferable resistance genes in these clinical isolates.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Serratia marcescens is an opportunistic pathogen.
  • Pandrug-resistant (PDR) strains pose a significant clinical challenge.
  • Understanding resistance mechanisms is crucial for treatment.

Purpose of the Study:

  • To determine the draft genome sequences of two PDR Serratia marcescens clinical isolates.
  • To investigate the genetic basis of the observed pandrug-resistance phenotype.

Main Methods:

  • Whole-genome sequencing of two clonal Serratia marcescens isolates.
  • Plasmid analysis to identify the location of resistance genes.

Main Results:

  • Draft genome sequences for two pandrug-resistant Serratia marcescens isolates were obtained.
  • The resistance phenotype was confirmed to be plasmid-mediated.
  • 14 out of 17 identified antibiotic resistance genes were located on large plasmids (IncFIB(K), IncHI2, and IncA/C2).

Conclusions:

  • The study identified a substantial pool of transmissible antibiotic resistance genes within these clinical isolates.
  • Plasmid-driven resistance in Serratia marcescens contributes to the spread of antimicrobial resistance.
  • Genomic insights into PDR strains are essential for infection control and therapeutic strategies.

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