Related Experiment Video

Updated: Mar 8, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

11.5K

TRIP13 is expressed in colorectal cancer and promotes cancer cell invasion

Kenji Kurita1, Masao Maeda2, Mohammed A Mansour2

  • 1Department of Surgical Oncology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.

Oncology Letters
|January 21, 2017
PubMed

Insights

Thyroid hormone receptor interactor 13 (TRIP13) is crucial in colorectal cancer (CRC) progression. Inhibiting TRIP13 in CRC cells reduced proliferation and invasion, suggesting TRIP13 as a potential therapeutic target for CRC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Thyroid hormone receptor interactor 13 (TRIP13) is an evolutionarily conserved protein involved in the ATPases associated with various cellular activities (AAA) family.
  • TRIP13 plays a critical role in the spindle assembly checkpoint inactivation and is implicated in various cancer progressions.

Purpose of the Study:

  • To investigate the role of TRIP13 in colorectal cancer (CRC).
  • To determine if TRIP13's catalytic activity is essential for cancer progression.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) to assess TRIP13 mRNA expression in CRC tissues.
  • Depletion of TRIP13 in CRC cell lines to evaluate its effect on proliferation, migration, and invasion.
  • Expression of a catalytically inactive TRIP13 mutant in CRC cells to assess the role of its enzymatic activity.

Main Results:

  • TRIP13 mRNA was significantly overexpressed in multiple CRC tissues.
  • Depletion of TRIP13 suppressed CRC cell proliferation, migration, and invasion.
  • Expression of an inactive TRIP13 mutant markedly reduced cancer cell invasion compared to wild-type TRIP13.

Conclusions:

  • TRIP13 is highly expressed in colorectal cancer and promotes tumor progression.
  • TRIP13's catalytic activity is essential for its role in CRC invasion.
  • TRIP13 represents a potential therapeutic target for colorectal cancer treatment.
Keywords:
AAA+TRIP13colorectal cancerinvasionmigration

More Related Videos

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

7.1K
In vitro Organoid Culture of Primary Mouse Colon Tumors07:33

In vitro Organoid Culture of Primary Mouse Colon Tumors

Published on: May 17, 2013

36.1K

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.4K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.7K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K