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Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

3.3K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

2.0K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
2.0K
Types Of Column Chromatography01:29

Types Of Column Chromatography

14.4K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
14.4K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.4K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
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Related Experiment Video

Updated: Mar 8, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Compact Ultrahigh-Pressure Nanoflow Capillary Liquid Chromatograph.

Xiaofeng Zhao, Xiaofeng Xie, Sonika Sharma

  • 1Tranxend LLC , 6550 South Millrock Drive, Suite 200, Salt Lake City, Utah 84121, United States.

Analytical Chemistry
|January 21, 2017
PubMed
Summary

A portable ultrahigh-pressure nanoflow liquid chromatograph (LC) system was developed for easy transport and coupling with mass spectrometry (MS). This compact LC system achieves high pressures and excellent reproducibility for efficient analysis.

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • Traditional liquid chromatography (LC) systems can be bulky and difficult to move.
  • There is a need for portable analytical instruments for on-site or flexible laboratory use.
  • Coupling LC with mass spectrometry (MS) requires robust and low-volume interfaces.

Purpose of the Study:

  • To develop a compact, portable ultrahigh-pressure nanoflow liquid chromatograph (LC) system.
  • To enable easy relocation and direct coupling with mass spectrometry (MS).
  • To achieve high-pressure operation and efficient sample analysis.

Main Methods:

  • Development of a compact LC system with integrated nanoflow pumps, low-volume injector, and mixing tee.
  • Operation at pressures up to 1100 bar (16000 psi) with a total pump capacity of 60 μL.
  • Evaluation of gradient performance, reproducibility using polycyclic aromatic hydrocarbons, and LC-MS coupling with a trypsin-digested bovine serum albumin sample.

Main Results:

  • The developed LC system is lightweight (4.5-5.9 kg) and capable of ultrahigh pressures.
  • Excellent day-to-day gradient accuracy (RSD 0.6%) and retention-time reproducibility (RSD < 0.17%) were achieved.
  • Successful LC-MS coupling demonstrated narrow peaks, short dwell times, and good peptide coverage.

Conclusions:

  • The compact ultrahigh-pressure nanoflow LC system is suitable for portable applications and direct MS coupling.
  • The system offers high performance in terms of pressure, reproducibility, and analysis time.
  • This portable LC-MS solution facilitates efficient analysis of complex biological samples.