Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

17.5K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
17.5K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.6K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Nuclear Transporter Transportin-3 Functions Under Oxidative Stress.

Cells·2026
Same author

Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein.

Nature communications·2025
Same author

AOP Report: Decreased ALDH1A (RALDH) activity leading to decreased fertility via disrupted meiotic initiation of fetal oogonia.

Current research in toxicology·2025
Same author

Ovarian development is driven by early spatiotemporal priming of the coelomic epithelium.

bioRxiv : the preprint server for biology·2025
Same author

A Pilot, Randomised, Placebo-Controlled, Double-Blind Trial of a Single Oral Dose of Ivermectin for Post-Exposure Prophylaxis of SARS-CoV-2.

Pharmaceutics·2025
Same author

The nuclear transport factor IPO5 revealed as a critical mediator of male germline development†.

Biology of reproduction·2025
Same journal

Modeling and analysis of forward and inverse kinematics for a flexible Stewart platform.

PloS one·2026
Same journal

Barriers and facilitators to healthcare utilization amongst people living with sickle cell disease in the United States: A scoping review.

PloS one·2026
Same journal

Enhancing data completeness in time series: Imputation strategies for missing data using significant periodically correlated components.

PloS one·2026
Same journal

Key targets and mechanisms by which gut microbiota-derived metabolites regulate Alzheimer's disease through the immune - inflammatory pathway: Based on network pharmacology and molecular docking.

PloS one·2026
Same journal

Grid-tied Transformer-less Boost Switched Capacitor Topology (TLBSCT) for PV applications.

PloS one·2026
Same journal

The load-velocity profiles and exercise-specific velocity zones for seven commonly used weightlifting exercises.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Mar 8, 2026

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

16.5K

Mice Lacking Hbp1 Function Are Viable and Fertile.

Cassy M Spiller1, Dagmar Wilhelm1, David A Jans2

  • 1Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia.

Plos One
|January 21, 2017
PubMed
Summary
This summary is machine-generated.

High mobility group box containing transcription factor 1 (HBP1) does not appear essential for fetal germ cell cycle arrest in mice. HBP1 loss-of-function mutants show normal germ cell proliferation and fertility, suggesting it

More Related Videos

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

8.9K
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

901

Related Experiment Videos

Last Updated: Mar 8, 2026

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

16.5K
A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

8.9K
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

901

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Molecular genetics

Background:

  • Fetal germ cell development is regulated by somatic cells and differs between sexes.
  • Germ cell cycle arrest (G1/G0) is crucial to prevent germ cell loss and tumors.
  • High mobility group box containing transcription factor 1 (HBP1) is a tumor suppressor in somatic cells and is present in fetal male germ cells.

Purpose of the Study:

  • To investigate the role of HBP1 in initiating and maintaining fetal germ cell G1/G0 arrest in mice.
  • To understand the function of HBP1 splice variants in gonadal development.

Main Methods:

  • Utilized mouse models, including embryonic stem (ES) cells with an Hbp1 Genetrap mutation.
  • Generated Hbp1-LacZ reporter mouse lines to track gene expression.
  • Examined Hbp1 expression and function in both XY and XX fetal gonads.
  • Analyzed germ cell proliferation and fertility in Hbp1-deficient mice.
  • Investigated Hbp1 regulation in the Rb1-knockout model.

Main Results:

  • Identified two Hbp1 splice variants expressed in fetal gonads, with one localized to the nucleus.
  • Hbp1-genetrap mutant mice lacking HBP1 function exhibited normal germ cell proliferation.
  • Adult male HBP1-deficient mice were viable and fertile.
  • Unexpectedly found embryonic HBP1 expression in hair follicles, eyes, and limbs.
  • No evidence of HBP1 mis-regulation in the Rb1-knockout model.

Conclusions:

  • HBP1 is not essential for the initiation or maintenance of fetal germ cell G1/G0 arrest in mice.
  • The previously reported RB1-HBP1 interaction is likely not critical in the germline.
  • HBP1 has roles beyond germ cell development, as indicated by its expression in other embryonic tissues.