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Updated: Mar 8, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
Published on: September 6, 2019
Synthesis of an allergy inducing tetrasaccharide "4P-X"
Takashi Moriya1, Naoki Nagahata2, Rei Odaka1
1Enzymes and Pharmaceuticals Laboratory, GODO SHUSEI Co., Ltd., 250 Nakahara, Kamihongo, Matsudo City, Chiba, 271-0064, Japan.
Abstract:
4P-X (β-D-galactopyranosyl-(1 → 4)-β-D-galactopyranosyl-(1 → 6)-[β-D-galactopyranosyl-(1 → 4)]-β-D-glucopyranose) is included in galacto-oligosaccharides (GOSs) produced by β-galactosidase derived from Bacillus circulans. 4P-X has been known to induce particularly strong allergies. High purity 4P-X is essential for use as a standard to quantify the amount of 4P-X in GOSs; however, the isolation of high purity 4P-X has never been reported. In this study, we achieved the synthesis of 4P-X by a combination of organic and enzymatic chemical syntheses in a short time. This is the first report of isolated, high purity 4P-X.
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