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[Flow cytometric DNA analysis of frozen samples]
M Kawasaki1, K Sasaki, T Murakami
1Dept. of Pediatric Surgery, Saiseikai Shimonoseki General Hospital.
Gan No Rinsho. Japan Journal of Cancer Clinics
|October 1, 1989
Summary
Flow cytometric DNA analysis is feasible with frozen samples. Storing samples at 5°C, -80°C, or -195°C for two weeks did not significantly impact diagnostic and prognostic evaluations.
Area of Science:
- Biomedical Science
- Molecular Biology
- Clinical Diagnostics
Background:
- Flow cytometric DNA analysis is a crucial diagnostic tool.
- Increasing sample volumes necessitate effective sample storage strategies.
- Standardized protocols for preserving sample integrity during storage are essential.
Purpose of the Study:
- To evaluate the impact of different storage temperatures on flow cytometric DNA analysis.
- To determine optimal freezing conditions for preserving sample quality for diagnostic and prognostic purposes.
- To assess the feasibility of analyzing frozen samples in flow cytometry.
Main Methods:
- Samples were stored for two weeks at three different temperatures: 5°C, -80°C, and -195°C.
- Flow cytometric DNA analysis was performed on stored samples.
- Histograms generated from stored samples were compared to assess differences.
Main Results:
- No significant differences were observed in the histograms of samples stored at 5°C, -80°C, and -195°C.
- Sample integrity for flow cytometric DNA analysis was maintained across all tested storage conditions.
- The study demonstrated the reliability of frozen samples for subsequent analysis.
Conclusions:
- Frozen samples are suitable for flow cytometric DNA analysis.
- Storage at 5°C, -80°C, or -195°C does not compromise diagnostic or prognostic evaluations.
- This finding supports the implementation of frozen sample storage for flow cytometry workflows.