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Binding induced strand displacement amplification for homogeneous protein assay
Yubin Li1, Sheng Liu1, Zike Zhao1
1College of Science, Guangdong Ocean University, Zhanjiang 524088, PR China.
Talanta
|January 22, 2017
Summary
A novel protein assay uses binding-induced strand displacement amplification (BI-SDA) for ultrasensitive detection. This method accurately quantifies platelet-derived growth factor (PDGF BB) with high sensitivity.
Area of Science:
- Biochemistry
- Molecular Biology
- Analytical Chemistry
Background:
- Ultrasensitive protein detection is crucial for early disease diagnosis.
- Existing assays often lack sensitivity or require complex procedures.
Purpose of the Study:
- To develop an ultrasensitive and homogenous strategy for protein detection.
- To establish a novel assay for platelet-derived growth factor BB (PDGF BB).
Main Methods:
- Established a binding-induced strand displacement amplification (BI-SDA) assay.
- Utilized aptamers for specific PDGF BB recognition.
- Employed a G-quadruplex DNA structure for fluorescence reporting with N-methyl porphyrin propionic acid IX (NMM).
Main Results:
- Achieved a detection limit of 3.6 pmol/L for PDGF BB.
- Demonstrated a linear detection range from 1.0 x 10-11 mol/L to 2.0 x 10-9 mol/L.
- Exhibited good selectivity and practicality for protein quantification.
Conclusions:
- The BI-SDA strategy offers a highly sensitive and homogenous method for protein detection.
- The assay shows potential for application in quantifying other protein biomarkers.
Keywords:
Binding-induced strand displacement amplificationHomogeneous assayPlatelet-derived growth factorProtein
