DNA fingerprinting by means of a nonradioactive probe of sulfonated DNA

A DNA fingerprinting technique that uses a cold sulfonated DNA probe is described. DNA was extracted from blood samples of a family, who were subjected to DNA fingerprinting. The restriction fragments that were hybridized with the probe were colorimetrically detected by an enzyme immunoassay. For DNA detection and labeling a commercially available kit was employed. The sensitivity of this fingerprint detection method has been proven comparable to detection by the radioactive method, and hence is easier to use in conventional laboratories.