A novel mitochondria-targeting fluorescent probe for hydrogen sulfide in living cells
Wei Shi1, Miaobo Pan1, Hao Qiang1
1Center of Drug Discovery, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
Abstract:
A novel mitochondria-targeting fluorescent probe compound S-N3 for hydrogen sulfide (H2 S) in living cells has been designed and synthesized in this study. This article contained the chemosynthesis and some studies on bioactivity of the target compound in living cells. Compound S-N3 is easy to synthesize and can remain stable under the effect of pH, system and photostability. In addition, it shows low cytotoxicity in cell imaging. And it can react with H2 S highly selective in PBS or FBS solution, which would cause the obvious increase in fluorescence intensity. Therefore, the low-cost detection method for H2 S is allowed for monitoring the quantity of H2 S existed in the mitochondria.
