Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wild Boar (<i>Sus scrofa</i>) as Reservoir of Pathogenic and Intermediate <i>Leptospira</i>.

Animals : an open access journal from MDPI·2026
Same author

Acetate and combinations of short-chain fatty acids increase oxidative phenotype and contribute to muscle fiber type shift in myotubes.

Frontiers in physiology·2026
Same author

Multipotent adult progenitor cell therapy: Effect of timing and frequency on lung health in preterm lambs during inflammation.

Pharmacological research·2025
Same author

Seroprevalence of 16 <i>Leptospira</i> Serovars in Wild Boar (<i>Sus scrofa</i>) Hunted in Saxony-Anhalt, Germany.

Animals : an open access journal from MDPI·2025
Same author

Quercetin, Kaempferol and Capsaicin Counteract the TGF-β1-Induced Upregulation of αSMA and Collagen in Myoblasts.

International journal of molecular sciences·2025
Same author

Nutritional strategies targeting age-related skeletal muscle fibrosis: underlying mechanisms.

Critical reviews in food science and nutrition·2025

Related Experiment Video

Updated: Mar 8, 2026

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K

Silver nanoparticles induce hormesis in A549 human epithelial cells.

Mireille M J P E Sthijns1, Waluree Thongkam2, Catrin Albrecht2

  • 1Department of Pharmacology and Toxicology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands.

Toxicology in Vitro : an International Journal Published in Association with BIBRA
|January 23, 2017
PubMed
Summary

Silver nanoparticles (AgNPs) exhibit hormetic effects, inducing cellular protection against toxicity at low doses. This adaptive response in human epithelial cells differs mechanistically from acrolein adaptation, with glutathione playing no role.

Keywords:
AcroleinGSHHormesisNrf2Silver nanoparticles

More Related Videos

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
05:50

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

Published on: June 2, 2023

2.0K
Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks
04:53

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

Published on: May 26, 2023

1.8K

Related Experiment Videos

Last Updated: Mar 8, 2026

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice
07:48

A Silver Nanoparticle Method for Ameliorating Biliary Atresia Syndrome in Mice

Published on: October 13, 2018

8.1K
Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles
05:50

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

Published on: June 2, 2023

2.0K
Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks
04:53

Author Spotlight: Advances in Evaluating Human Lung Epithelial Cells' Response to Metal-Organic Frameworks

Published on: May 26, 2023

1.8K

Area of Science:

  • Nanotechnology
  • Toxicology
  • Cell Biology

Background:

  • Silver nanoparticles (AgNPs) are increasingly used in medicine, food, and consumer products.
  • Knowledge gaps exist regarding AgNP toxicity and cellular responses to prolonged exposure.
  • Prior exposure may confer cellular resistance to AgNPs, suggesting a hormetic response.

Purpose of the Study:

  • To investigate if silver nanoparticles (AgNPs) induce a hormetic response in human epithelial cells.
  • To compare the adaptive mechanisms of AgNPs with that of acrolein.
  • To determine the role of glutathione (GSH) in AgNP-induced adaptation.

Main Methods:

  • A549 human epithelial cells were pretreated with two types of AgNPs (colloidal and powder) at low doses.
  • Cells were subsequently exposed to toxic doses of AgNPs and acrolein.
  • Mechanisms investigated included Nrf2 translocation, mRNA expression of γGCS and HO-1, and GSH levels.

Main Results:

  • Low-dose AgNP pretreatment protected cells against toxic doses of AgNPs and acrolein.
  • Protection was more significant with colloidal AgNPs.
  • AgNP-induced adaptation involved HO-1 mRNA expression and Nrf2 immunoreactivity, but not increased GSH levels or γGCS mRNA expression, unlike acrolein adaptation.

Conclusions:

  • Silver nanoparticles (AgNPs) induce a hormetic response, conferring cellular protection against toxicity.
  • The adaptive mechanism of AgNPs differs from that of acrolein, notably independent of glutathione.
  • Further research into the specific pathways of AgNP-induced adaptation is warranted.