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Related Experiment Video

Updated: Mar 8, 2026

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Optical Barcoding for Single-Clone Tracking to Study Tumor Heterogeneity.

Malte Mohme¹, Cecile L Maire¹, Kristoffer Riecken²

¹Laboratory for Brain Tumor Biology, Department of Neurosurgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.

Molecular Therapy : the Journal of the American Society of Gene Therapy

|January 23, 2017

Summary

This summary is machine-generated.

A new optical barcoding technique enables precise tracking of live cancer cell clones. This method aids in understanding tumor heterogeneity and resistance, offering a powerful tool for cancer research.

Keywords:

LeGO vectors barcoding clonal tracking flow cytometry fluorescent labeling glioma invasion in vivo mouse model tumor heterogeneity

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

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Published on: November 14, 2025

Area of Science:

Oncology
Biotechnology
Molecular Biology

Background:

Intratumoral heterogeneity drives cancer treatment resistance and recurrence.
Understanding clonal dynamics is crucial for effective cancer therapies.
Current methods for tracking cell clones are limited.

Purpose of the Study:

To develop a novel fluorescent optical barcoding technique for tracking live cell clones.
To enable rapid, sensitive, and quantitative analysis of clonal composition.
To investigate clonal contributions to glioblastoma growth in vivo.

Main Methods:

Developed a fluorescent optical barcoding method using six fluorescent proteins and three colors.
Applied the technique to two malignant glioma cell lines for in vitro and in vivo studies.
Utilized flow cytometry (FC) for high-throughput analysis and fluorescence-activated cell sorting (FACS) for clone recovery.

Main Results:

Demonstrated unambiguous barcoding of up to 41 distinct clones.
Showcased the technique's sensitivity, precision, speed, and cost-effectiveness.
Quantified the contribution of multiple clones to glioblastoma tumor growth in vivo.

Conclusions:

Optical barcoding is a powerful tool for tracking clonal cell populations in vitro and in vivo.
This technique significantly advances the study of tumor heterogeneity and clonal dynamics.
Enables new strategies for developing targeted cancer therapies.