Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Activated macrophages and cytotoxicity].

Y Yamamura

    Kekkaku : [Tuberculosis]
    |October 1, 1989
    PubMed
    Summary
    This summary is machine-generated.

    Cellular immunity in tuberculosis involves activated macrophages releasing tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). Tuberculin-positive individuals, including those with tuberculosis, show higher TNF-alpha production from macrophages.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Distinct downstream signaling mechanism between erythropoietin receptor and interleukin-2 receptor.

    The EMBO journal·1992
    Same author

    Pharmacokinetic profile of glycyrrhizin in healthy volunteers by a new high-performance liquid chromatographic method.

    Journal of pharmaceutical sciences·1992
    Same author

    Metabolism of subcutaneous administered indium arsenide in the hamster.

    Toxicology and applied pharmacology·1992
    Same author

    Perinatal adaptation of the cardiovascular system.

    Early human development·1992
    Same author

    Characterization of a novel aquaretic agent, OPC-31260, as an orally effective, nonpeptide vasopressin V2 receptor antagonist.

    British journal of pharmacology·1992
    Same author

    Identification and characterization of specific receptors for the LD78 cytokine.

    International journal of hematology·1992

    Area of Science:

    • Immunology
    • Cell Biology
    • Pathology

    Context:

    • Cellular immune responses are crucial in managing tuberculous infections.
    • Activated macrophages accumulate at infection sites, phagocytosing bacteria but also causing tissue damage through enzyme and cytokine release.
    • Understanding macrophage-mediated necrosis is key to tuberculosis pathogenesis.

    Purpose:

    • To investigate the production of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) by human monocytes/macrophages (M phi) in response to stimulation.
    • To compare cytokine production in individuals with varying tuberculin reactivity and tuberculosis status.

    Summary:

    • Human monocytes/macrophages were isolated from healthy tuberculin-negative, healthy tuberculin-positive, and tuberculosis patients.
    • Cells were stimulated with lipopolysaccharide (LPS) or muramyl dipeptide (MDP), and TNF-alpha and IL-6 levels in supernatants were measured.

    Related Experiment Videos

  • Monocytes/macrophages from tuberculin-positive individuals (with or without TB) exhibited higher TNF-alpha production compared to tuberculin-negative individuals.

    • Impact:

    • Findings suggest a link between cellular immune status, macrophage activation, and cytokine profiles in tuberculosis.
    • Macrophage-derived TNF-alpha may play a significant role in the inflammatory and necrotic processes observed in tuberculosis.
    • This research contributes to understanding the immunological mechanisms underlying tissue damage in tuberculosis.