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Related Experiment Videos

Explosive Blast Loading on Human 3D Aggregate Minibrains.

Nicole E Zander1, Thuvan Piehler2, Helena Hogberg3

  • 1Department of the Army, US Army Research Laboratory, Weapons and Materials Research Directorate, RDRL-WMM-G, Building 4600, Aberdeen Proving Ground, MD, 21005, USA. nicole.e.zander.civ@mail.mil.

Cellular and Molecular Neurobiology
|January 23, 2017
PubMed
Summary
This summary is machine-generated.

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Primary explosive blasts can damage brain cells, increasing oxidative stress with higher pressures. However, this study found minimal cell death in a 3D human brain model exposed to military explosives.

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Toxicology

Background:

  • The impact of primary explosive blasts on brain tissue is poorly understood.
  • Limited in vitro models exist to study explosive blast injury mechanisms at the cellular level.

Purpose of the Study:

  • To investigate the cellular effects of primary explosive blasts on human brain tissue using a novel in vitro model.
  • To determine the relationship between blast pressure and the extent of brain tissue injury.

Main Methods:

  • Utilized a 3D brain microphysiological system (MPS) composed of human brain cells.
  • Exposed the 3D brain cell aggregates to military explosives at controlled pressures of 50 and 100 psi.

Main Results:

Keywords:
ExplosivesIn vitro modelInduced pluripotent stem cellsMinibrainPrimary blastTraumatic brain injury

Related Experiment Videos

  • Exposure to explosive blasts resulted in increased membrane damage in brain cells.
  • Oxidative stress markers were elevated in response to increasing blast pressure.
  • Despite observed damage and stress, overall cell death remained minimal.

    • Conclusions:

    • The 3D brain microphysiological system is a viable model for studying explosive blast effects on neural tissue.
    • Primary explosive blasts induce cellular stress and membrane damage in brain tissue, with severity correlating to pressure.
    • Further research is needed to understand the long-term implications of minimal cell death following blast exposure.