Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.8K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.8K
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

3.9K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
3.9K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

1.2K
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
1.2K
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

4.0K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
4.0K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.3K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.3K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

749
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
749

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial complex I activity in microglia sustains neuroinflammation.

Nature·2024
Same author

Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation.

bioRxiv : the preprint server for biology·2024
Same author

The use of biomarkers to stratify surgical care in women with ovarian cancer: Scientific Impact Paper No. 69 May 2022.

BJOG : an international journal of obstetrics and gynaecology·2022
Same author

Evaluation of post-exposure prophylaxis practices to improve the cost-effectiveness of rabies control in human cases potentially exposed to rabies in southern Bhutan.

BMC infectious diseases·2020
Same author

Correction to: Assessing the effect of nitisinone induced hypertyrosinaemia on monoamine neurotransmitters in brain tissue from a murine model of alkaptonuria using mass spectrometry imaging.

Metabolomics : Official journal of the Metabolomic Society·2019
Same author

Assessing the effect of nitisinone induced hypertyrosinaemia on monoamine neurotransmitters in brain tissue from a murine model of alkaptonuria using mass spectrometry imaging.

Metabolomics : Official journal of the Metabolomic Society·2019
Same journal

Cancer immunotherapy with oncolytic myxoma virus.

Advances in cancer research·2026
Same journal

Cancer terminator viruses: Unique conditionally replication competent oncolytic viruses producing therapeutic anti-cancer immunomodulating agents.

Advances in cancer research·2026
Same journal

Advancing clinical translation of oncolytic adenoviruses.

Advances in cancer research·2026
Same journal

Operation oncolysis: Reoviruses in the theater of cancer war.

Advances in cancer research·2026
Same journal

AAV vectors for cancer gene therapy.

Advances in cancer research·2026
Same journal

Vesicular stomatitis virus (VSV) as an oncolytic therapeutic agent for cancer.

Advances in cancer research·2026
See all related articles

Related Experiment Video

Updated: Mar 8, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.6K

Ambient Mass Spectrometry in Cancer Research.

Z Takats1, N Strittmatter2, J S McKenzie1

  • 1Imperial College London, London, United Kingdom.

Advances in Cancer Research
|January 24, 2017
PubMed
Summary
This summary is machine-generated.

Ambient ionization mass spectrometry, including DESI-MS and REIMS, offers sample preparation-free analysis for unaltered biological tissues. These techniques enable real-time intraoperative tissue identification and lipidomic phenotyping, advancing cancer research and surgical assessment.

Keywords:
Ambient mass spectrometryDesorption electrospray mass spectrometry imagingDrug imagingIn vivo mass spectrometryMargin assessmentRapid evaporative ionization mass spectrometry

More Related Videos

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.6K
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.2K

Related Experiment Videos

Last Updated: Mar 8, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.6K
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
10:42

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Published on: May 12, 2023

1.6K
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.2K

Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Mass Spectrometry

Background:

  • Ambient ionization mass spectrometry (MS) provides sample preparation-free analysis, contrasting with traditional workflows.
  • Desorption electrospray ionization (DESI)-MS allows direct analysis of diverse samples, including biological tissues.
  • DESI-MS is predominantly used for mass spectrometric imaging (MSI) in cancer research, offering matrix-free experiments and improved low m/z signal.

Purpose of the Study:

  • To highlight DESI-MSI's capabilities as an alternative to MALDI for matrix-free MSI.
  • To showcase DESI-MSI's utility in mapping tumor metabolism and serving as a histology alternative for intraoperative tissue identification.
  • To introduce Rapid Evaporative Ionization Mass Spectrometry (REIMS) for real-time intraoperative tissue analysis and lipidomic phenotyping.

Main Methods:

  • Desorption electrospray ionization mass spectrometry (DESI-MS) for direct tissue analysis and imaging.
  • Mass spectrometric imaging (MSI) for spatial mapping of tumor metabolism.
  • Rapid Evaporative Ionization Mass Spectrometry (REIMS) coupled with electrosurgical devices for aerosol analysis.

Main Results:

  • DESI-MSI provides matrix-free experiments with enhanced signal in the lower m/z range (<500 m/z).
  • DESI-MSI facilitates intraoperative tissue identification and surgical margin assessment, acting as an alternative to frozen section histology.
  • REIMS offers real-time histological information based on tissue lipid composition, proving effective for intraoperative margin control and rapid lipidomic phenotyping of cell lines.

Conclusions:

  • Ambient ionization MS techniques like DESI-MS and REIMS offer significant advantages for direct tissue analysis and intraoperative applications.
  • DESI-MSI and REIMS are valuable tools for cancer research, surgical guidance, and rapid phenotyping.
  • These methods provide real-time, detailed molecular information from biological samples with minimal sample preparation.