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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Related Experiment Video

Updated: Mar 8, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Applications of Mass Spectrometry Imaging to Cancer.

G Arentz1, P Mittal1, C Zhang1

  • 1Adelaide Proteomics Centre, School of Biological Sciences, University of Adelaide, Adelaide, SA, Australia; Institute for Photonics and Advanced Sensing (IPAS), University of Adelaide, Adelaide, SA, Australia.

Advances in Cancer Research
|January 24, 2017
PubMed
Summary

Mass spectrometry offers label-free, spatial molecular analysis for pathology. This technology aids in diagnosing tumors, understanding their environment, and improving patient treatment decisions.

Keywords:
CancerClassificationImagingMALDIMass spectrometryPrediction

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Last Updated: Mar 8, 2026

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Area of Science:

  • Pathology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Pathologists diagnose tumors using tissue samples and microscopic examination.
  • Current methods rely on visual interpretation of stained tissue sections.
  • Targeted protein analysis provides additional clinical data.

Purpose of the Study:

  • To introduce novel mass spectrometry techniques for pathology.
  • To enable label-free, spatial molecular profiling of tissue specimens.
  • To enhance tumor diagnosis, prognosis, and treatment strategies.

Main Methods:

  • Acquisition of mass spectra at each pixel (x/y) of tissue sections.
  • Generation of label-free, non-targeted molecular intensity maps.
  • Analysis of proteins, peptides, lipids, small molecules, or glycans.

Main Results:

  • Mass spectrometry provides unbiased molecular information across tissue sections.
  • Intensity maps visualize the spatial distribution of molecules.
  • Techniques allow characterization of tumor cells and their microenvironment.

Conclusions:

  • Mass spectrometry-based methods offer new insights into tumor tissues.
  • These techniques complement histological staining for improved diagnosis and management.
  • This approach has the potential to transform pathology services globally.