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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Aptamers: Universal capture units for lateral flow applications.

Christin Fischer¹, Hauke Wessels¹, Angelika Paschke-Kratzin¹

¹Hamburg School of Food Science; Institute of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.

Analytical Biochemistry

|January 24, 2017

Summary

This summary is machine-generated.

Aptamers successfully function as capture molecules in lateral flow assays for diverse targets, including metabolites, proteins, and spores. This demonstrates their potential as a versatile alternative to antibodies in diagnostic applications.

Keywords:

Aptamers Capture molecules Lateral flow Lysozyme Metabolite Small molecules Spores

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Area of Science:

Biotechnology
Analytical Chemistry
Biosensors

Background:

Antibodies are traditionally used as capture molecules in lateral flow assays.
The need for antibody alternatives is driven by ethical considerations and the desire for versatile detection platforms.
Aptamers offer a promising alternative due to their stability, specificity, and ease of synthesis.

Purpose of the Study:

To demonstrate the efficacy of aptamers as capture molecules in lateral flow assays across various target classes.
To establish a universal lateral flow test platform utilizing aptamers.
To evaluate aptamers as a viable alternative to antibodies in line with animal protection directives.

Main Methods:

Selection and implementation of aptamers for target molecules representing different size classes: p-aminohippuric acid (metabolite), lysozyme (protein), and Alicyclobacillus spores (whole cell).
Development of lateral flow assay (LFA) platforms incorporating these aptamers as capture agents.
Determination of the limit of detection (LOD) for each target analyte using the aptamer-based LFAs.

Main Results:

Successful implementation of aptamers as capture molecules in LFAs, irrespective of target size.
Demonstrated detection limits: p-aminohippuric acid in urine (200 ppm), lysozyme in white wine (20 ppm), and Alicyclobacillus spores in orange juice (>8 CFU/mL).
Proof-of-concept validation for aptamers in a universal LFA platform.

Conclusions:

Aptamers are effective capture molecules for a broad range of targets in lateral flow assay applications.
Aptamer-based LFAs provide a versatile and potentially more ethical alternative to traditional antibody-based assays.
This study supports the broader adoption of aptamers in diagnostic and analytical testing platforms.