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Updated: Mar 8, 2026
Synergetic Use of Neural Precursor Cells and Self-assembling Peptides in Experimental Cervical Spinal Cord Injury
Published on: February 23, 2015
Introduction of neurosupportive cells into processed acellular nerve allografts results in greater number and more
Matthew J Thompson1, Gaurangkumar Patel1, Jonathan Isaacs1
1a Division of Hand Surgery, Department of Orthopaedic Surgery , Virginia Commonwealth University Medical Center , Richmond , VA , USA.
Objectives:
Processing necessary to remove immunogenic components of nerve allograft renders it acellular. Seeding with supportive cells may improve axon regeneration. We aim to identify the method associated with implantation of the greatest volume and most even distribution of cells.
Methods:
Hypodermic needle injection was compared to soaking in solution under both normal and pressurized conditions after micropuncture of the allograft. Distribution within the allograft was measured using an in vitro model of fluorescent beads, as well as cultured Schwann cells.
Results:
Injection treatment resulted in larger volumes and a more uniform cross-sectional distribution of implanted cells. Beads and cells behaved similarly relative to the measured outcomes.
Conclusions:
Injection instills more cells in a more uniform distribution. In vivo testing may evaluate whether these techniques vary relative to cell survival, cell migration, and clinical outcomes. Size- and concentration-matched fluorescent beads may represent a viable model for analyzing cell implantation.
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