Effects of Trichostatin A and PXD101 on the In Vitro Development of Mouse Somatic Cell Nuclear Transfer Embryos
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Updated: Mar 8, 2026
Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
Published on: April 26, 2018
Xiaoyan Qiu1, Haihong You1, Xiong Xiao1
1Embryo Engineering Laboratory, School of Animal Science and Technology, Southwest University , Chong Qing, P.R. China .
Abstract:
The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with aberrant epigenetic nuclear reprogramming. It has been demonstrated that treatment with histone deacetylase inhibitors (HDACis) enhances developmental potential of SCNT embryos. Previous studies in many species revealed that treatment of SCNT embryos with trichostatin A (TSA)-an HDACi-significantly enhances the in vitro development of SCNT embryos. In this study, we compared two different SCNT protocols with TSA and investigate, for the first time, the effect of another new HDACi, PXD101 (belinostat), on in vitro development of mouse SCNT embryo. Rates of blastocyst development in mouse SCNT embryos treated with either 5 nM TSA during (6 hours) and after (4 hours) activation (39.1%) or with 50 nM PXD101 during (6 hours) and after (4 hours) activation (40.2%) were significantly higher than those of nontreated SCNT embryos (11.5%) and both treatments also significantly improved the subsequent establishment of NT-ESCs in comparison with the nontreated group (38.1% and 40.9% vs. 11.8%). In conclusion, we optimized the TSA concentration and treatment timing and, for the first time, investigated the effect of PXD101 on mouse development of SCNT embryos and establishment of NT-ESCs.
Insights
Histone deacetylase inhibitors (HDACis) improve animal cloning. This study found that trichostatin A (TSA) and PXD101 significantly enhance mouse somatic cell nuclear transfer (SCNT) embryo development and stem cell establishment.
Area of Science:
- Reproductive biology
- Developmental biology
- Epigenetics
Background:
- Somatic cell nuclear transfer (SCNT) cloning has low success rates due to epigenetic reprogramming issues.
- Histone deacetylase inhibitors (HDACis) are known to improve SCNT embryo development.
- Trichostatin A (TSA) is a known HDACi that enhances SCNT embryo development in various species.
Purpose of the Study:
- To compare the efficacy of two different SCNT protocols using TSA.
- To investigate the effect of a new HDACi, PXD101 (belinostat), on mouse SCNT embryo development for the first time.
- To optimize TSA concentration and treatment timing for SCNT.
Main Methods:
- Mouse SCNT embryos were treated with either TSA (5 nM) or PXD101 (50 nM) during and after activation.
- Developmental rates to blastocyst stage were assessed.
- The ability to establish nuclear transfer-derived embryonic stem cells (NT-ESCs) was evaluated.
Main Results:
- TSA treatment resulted in a 39.1% blastocyst development rate, while PXD101 treatment yielded 40.2%.
- Both TSA and PXD101 treatments significantly increased blastocyst rates compared to non-treated SCNT embryos (11.5%).
- NT-ESC establishment rates were significantly higher in both treatment groups (38.1% for TSA, 40.9% for PXD101) compared to the control group (11.8%).
Conclusions:
- Optimized TSA concentration and treatment timing enhance mouse SCNT embryo development.
- PXD101 significantly improves in vitro development of mouse SCNT embryos and NT-ESC establishment.
- HDACis represent a promising strategy for improving SCNT efficiency and reprogramming in mammals.
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