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Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Published on: December 23, 2014
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Nuclear Glycogen Inclusions in Canine Parietal Cells
S Silvestri1, E Lepri1, C Dall'Aglio1
11 Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
Veterinary Pathology
|January 24, 2017
Summary
Nuclear glycogen inclusions were found in canine parietal cells, a novel observation. These inclusions, confirmed as glycogen, appear to be an incidental finding without association with gastritis.
Area of Science:
- Veterinary Pathology
- Cell Biology
- Gastroenterology
Background:
- Nuclear glycogen inclusions are rare and their significance is not well understood.
- Previous reports of nuclear glycogen inclusions in canine tissues are absent.
- Parietal cells are key components of the gastric mucosa.
Purpose of the Study:
- To investigate the presence and composition of intranuclear inclusions in canine parietal cells.
- To determine if these inclusions are glycogen and if they are associated with specific conditions.
Main Methods:
- Retrospective examination of canine gastric mucosa samples.
- Histologic techniques including periodic acid-Schiff (PAS) and diastase staining.
- Transmission electron microscopy with periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining.
Main Results:
- Intranuclear inclusions/pseudoinclusions were identified in 22% of examined canine gastric mucosa samples.
- Inclusions stained positive with PAS and were diastase-sensitive, confirming glycogen.
- Ultrastructural analysis confirmed glycogen composition and suggested in situ synthesis, with no association to gastritis.
Conclusions:
- Nuclear glycogen inclusions are a newly identified finding in canine parietal cells.
- These inclusions appear to be incidental and not linked to gastritis or Helicobacter-like organisms.
- Further research is needed to elucidate the precise function and significance of these nuclear glycogen deposits.
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