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Area of Science:

  • Vascular Biology
  • Cellular Physiology
  • Pathophysiology

Background:

  • Decompression sickness (DCS) involves bubble formation and systemic effects.
  • Endothelial microparticles (EMPs) are key indicators of endothelial function and vascular health.
  • The specific impact of bubble-induced EMPs on endothelial cells requires further investigation.

Purpose of the Study:

  • To investigate the effects of bubble-induced EMPs on endothelial cells in vitro and in vivo.
  • To explore the potential of a surfactant (FSN-100) in mitigating these effects.
  • To understand the role of EMPs in DCS pathogenesis.

Main Methods:

  • Isolated rat pulmonary microvascular endothelial cells (PMVECs) and stimulated them with bubbles.
  • Collected and incubated bubble-induced EMPs with normal PMVECs, assessing cell viability, apoptosis, permeability, and cytokine release.
  • Administered bubble-induced EMPs intravenously to rats to evaluate endothelial dysfunction markers in vivo.

Main Results:

  • Bubble stimulus significantly increased EMP release (3-fold).
  • Bubble-induced EMPs reduced cell viability, increased apoptosis, cell permeability, and pro-inflammatory cytokine expression.
  • In vivo, EMPs elevated markers of endothelial dysfunction, effects attenuated by FSN-100 pretreatment.

Conclusions:

  • Bubble-induced EMPs mediate endothelial dysfunction both in vitro and in vivo.
  • An EMP abatement strategy using FSN-100 shows promise in mitigating these detrimental effects.
  • These findings highlight the significant role of bubble-induced EMPs in DCS pathology.