Related Experiment Video
Updated: Mar 8, 2026
07:55
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
Published on: June 26, 2020
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PHF11 promotes DSB resection, ATR signaling, and HR
Yi Gong1, Naofumi Handa2,3, Stephen C Kowalczykowski2,3
1Laboratory for Cell Biology and Genetics, The Rockefeller University, New York, New York 10065, USA.
Genes & Development
|January 25, 2017
Summary
PHF11 is a newly discovered DNA damage response factor crucial for 5' end resection and homologous recombination repair of double-strand breaks (DSBs). Its function promotes DNA repair signaling and genomic stability.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- DNA double-strand breaks (DSBs) require precise repair mechanisms.
- Resection of DSBs generates single-stranded DNA (ssDNA) that activates DNA damage response (DDR) pathways, including ATR signaling and homologous recombination (HR).
Purpose of the Study:
- To identify and characterize novel factors involved in DSB resection and DDR.
- To elucidate the role of PHF11 in DNA damage response and repair.
Main Methods:
- Yeast-two hybrid screening
- Immunofluorescence microscopy
- siRNA-mediated knockdown
- Comet assay
- Western blotting
- Co-immunoprecipitation
- In vitro biochemical assays
Main Results:
- PHF11 (plant homeodomain finger 11) was identified as a DDR factor associated with deprotected telomeres and DNA damage sites.
- PHF11 depletion impaired ATR signaling, reduced 5' end resection, compromised HR, and led to misrejoining of DSBs.
- PHF11 interacts with RPA and stimulates EXO1 activity, promoting resection at RPA-bound DNA damage sites.
Conclusions:
- PHF11 is a novel regulator of 5' end resection, essential for ATR signaling and homologous recombination repair.
- PHF11's function in promoting EXO1 activity is critical for efficient DSB repair and maintaining genomic integrity.
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