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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

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STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing

Shengjun Li1, Kan Liu1, Shuxin Zhang1,2

  • 1Center for Plant Science Innovation & School of Biological Sciences, University of Nebraska, Lincoln, NE 68588-0660.

Proceedings of the National Academy of Sciences of the United States of America
|January 25, 2017
PubMed

Insights

SHORT VALVE 1 (STV1), a ribosomal protein, regulates microRNA (miRNA) levels in Arabidopsis. STV1 binds pri-miRNAs to promote their processing and influences transcription of miRNA genes, refining miRNA accumulation.

Area of Science:

  • Molecular Biology
  • Plant Science
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression, processed from primary miRNA transcripts (pri-miRNAs).
  • Accurate control of miRNA levels is essential for diverse biological processes.
  • DNA-dependent polymerase II (Pol II) transcribes most pri-miRNAs.

Purpose of the Study:

  • To investigate the role of SHORT VALVE 1 (STV1), a conserved ribosomal protein, in miRNA biogenesis.
  • To elucidate the mechanism by which STV1 influences miRNA levels in Arabidopsis.

Main Methods:

  • Localization studies to determine STV1's subcellular location.
  • RNA immunoprecipitation (RIP) to assess STV1 binding to pri-miRNAs.
  • Reporter assays using pri-miR172b to study STV1's function in processing.
  • Analysis of Pol II occupancy and promoter activity of miRNA genes (MIR).

Main Results:

  • STV1 localizes to the nucleus and binds directly to pri-miRNAs, specifically at the stem-loop region.
  • Loss of STV1 function impairs the association of pri-miRNAs with their processing complex.
  • STV1 indirectly affects Pol II occupancy at MIR gene promoters and influences their activity.
  • STV1 facilitates pri-miRNA recruitment to the processing complex, promoting miRNA biogenesis.

Conclusions:

  • STV1 plays a dual role in refining miRNA accumulation by enhancing pri-miRNA processing and influencing MIR gene transcription.
  • This study reveals a novel extraribosomal function for the ribosomal protein STV1 in regulating gene expression via miRNA biogenesis.
Keywords:
ArabidopsisSTV1 RNA bindingmiRNA biogenesispri-miRNAs

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