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Updated: Mar 8, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
Evaluation of rapid GeneXpert MTB/RIF method using DNA tissue specimens of vertebral bones in patients with suspected
Muhammad Nasrum Massi1, Karya Triko Biatko2, Irda Handayani3
1Department of Medical Microbiology, Medical Faculty, Hasanuddin University, Makassar 90245, Indonesia.
Purpose:
To detect Mycobacterium tuberculosis DNA and rifampicin resistance in vertebral bone tissue specimens from spondylitis TB suspects.
Methods And Results:
The rapid GeneXpert MTB/RIF and MGIT 960 liquid culture methods have been used in the specimens. Results from 70 suspects with spondylitis TB shown that 31.42% identified positive for spondylitis TB using culture method, while 88.57% shown positive results using rapid GeneXpert MTB/RIF method. The validity of GeneXpert MTB/RIF shown sensitivity value of 100%, specificity value of 16.6%, PPV of 35.48%, and NPV of 100%.
Conclusion:
GeneXpert has a high sensitivity but low specificity value in this study.

