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Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution
1School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People's Republic of China.
AMB Express
|January 25, 2017
Summary
Extractive fermentation using Triton X-100 (TX) efficiently produces Monascus pigments. Pigment secretion is limited by saturation concentration, with optimal extraction at low cell density and high TX levels.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Biochemistry
Background:
- Monascus pigments are valuable natural colorants.
- Extractive fermentation offers an efficient production method.
- Understanding pigment secretion mechanisms is crucial for optimization.
Purpose of the Study:
- To investigate pigment secretion behavior during extractive fermentation.
- To elucidate the role of nonionic surfactants, specifically Triton X-100 (TX), in pigment extraction.
- To explain pigment characteristic variations and extraction capacity.
Main Methods:
- Extractive fermentation using Monascus in a nonionic surfactant aqueous solution.
- Cultivation under varying cell densities and Triton X-100 concentrations.
- Analysis of intracellular and extracellular pigment levels.
Main Results:
- Pigment secretion is limited by saturation concentration in the surfactant solution.
- Complete pigment extraction requires low cell density and high TX concentration.
- Inconsistent changes in intracellular and extracellular pigments suggest pigment conversion during secretion.
- A potential enzyme-catalyzed conversion of orange to yellow pigment during transmembrane transport was inferred.
Conclusions:
- Extractive fermentation with TX is effective for Monascus pigment production.
- Cell density and TX concentration critically influence pigment extraction efficiency.
- Pigment characteristics can change during secretion, possibly via enzymatic conversion.
- This study provides insights into the mechanism of pigment variation and extraction in this process.
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