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Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI
Published on: August 18, 2017
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Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-Chip/Mass Spectrometry, and Functional Assays
Walmor C De Mello1, Yamil Gerena2
1School of Medicine, Medical Sciences Campus UPR, 365067, San Juan, PR, 00936, USA. walmor.de-mello@upr.edu.
Methods in Molecular Biology (Clifton, N.J.)
|January 25, 2017
Abstract:
The molecular mechanisms related to the effect of angiotensin II, its level on cardiac tissues, as well as its overexpression represent an important aspect of cardiovascular pharmacology and pathology. Severe alterations of cardiac functions are induced by hypertension including activation of circulating and local cardiac renin angiotensin systems. In this chapter, we are providing the methods and materials necessary for further investigation of this important topic.
Keywords:
AT1 receptorAngiotensin IICardiomyocytesELISAFlow cytometryHPLC-MSHeartIntracellularOverexpressionVisualization
