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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 8, 2026

Isolation and Characterization of Microvesicles from Peripheral Blood

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

Isolation and Characterization of Circulating Microparticles by Flow Cytometry.

Dylan Burger¹, Paul Oleynik²

¹Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada, K1H 8M5. dburger@uottawa.ca.

Methods in Molecular Biology (Clifton, N.J.)

|January 25, 2017

Summary

This study details a new protocol for isolating and quantifying microparticles, which are cell membrane fragments elevated in disease. The method uses flow cytometry for assessing microparticle levels in plasma and other biological samples.

Keywords:

Biomarker Ectosome Flow cytometry Membrane Microparticle Microvesicle Plasma

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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Area of Science:

Biochemistry
Cell Biology
Medical Diagnostics

Background:

Microparticles are cell membrane fragments (0.1-1.0 μm) released by stressed cells.
Elevated circulating microparticle levels are associated with various pathological conditions.
Accurate assessment and quantification are crucial for understanding disease states.

Purpose of the Study:

To describe a reliable protocol for isolating microparticles from plasma.
To detail a method for characterizing and quantifying microparticles using flow cytometry.
To provide an adaptable assay for diverse biological samples.

Main Methods:

Isolation of microparticles from plasma samples.
Characterization and quantification via flow cytometry.
Adaptation of the assay for cell culture media, urine, and other biological fluids.

Main Results:

A standardized protocol for microparticle isolation and quantification was established.
Flow cytometry enables precise characterization of microparticle populations.
The assay demonstrated applicability across human and animal plasma samples.

Conclusions:

The described flow cytometry protocol offers a robust method for microparticle assessment.
This assay is valuable for research in disease states associated with microparticle changes.
The protocol's adaptability supports broad applications in biological and clinical research.