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Isolation of Immune Cells for Adoptive Transfer
Tlili Barhoumi1, Pierre Paradis1, Koren K Mann1,2
1Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|January 25, 2017
Summary
Adoptive transfer of T regulatory cells (Treg cells) can help study immune system roles in hypertension and vascular disease. This method details isolating and purifying Treg cells from mouse spleens for transfer into recipient animals.
Area of Science:
- Immunology
- Cardiovascular Research
- Cellular Biology
Background:
- Hypertension and vascular diseases involve complex immune system interactions.
- T regulatory cells (Treg cells) play a crucial role in immune homeostasis and disease modulation.
- Characterizing Treg cell function requires robust isolation and transfer techniques.
Purpose of the Study:
- To describe a method for isolating and purifying T regulatory cells from mouse spleens.
- To provide a protocol for adoptive transfer of T regulatory cells for disease research.
- To facilitate the study of immune system involvement in hypertension and vascular disease.
Main Methods:
- Isolation of splenic T lymphocytes from donor mice.
- Processing spleen tissue to achieve a single-cell suspension.
- Utilizing negative and positive selection techniques to obtain CD4+ T cells and CD4+CD25+ Treg cells.
- Adoptive transfer of isolated Treg cells into recipient animals.
Main Results:
- Successful isolation of CD4+ T cells and CD4+CD25+ Treg cells.
- Demonstration of a viable protocol for Treg cell purification.
- Establishment of a method for Treg cell adoptive transfer.
Conclusions:
- The described method provides a reliable approach for obtaining functional Treg cells for adoptive transfer.
- This technique is valuable for investigating the immunological mechanisms underlying hypertension and vascular disease.
- Further studies can utilize this method to explore Treg cell-based therapies.
