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Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents
Qi-Bin Cheng1,2, Li-Wei Zhang3
1Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. qbcheng1992@163.com.
Molecules (Basel, Switzerland)
|January 25, 2017
Summary
This study introduces a green enzymatic method for producing daidzein from daidzin using deep eutectic solvents (DESs). The optimized process achieved a high daidzein yield and purity, demonstrating an eco-friendly and efficient production strategy.
Area of Science:
- Biotechnology
- Green Chemistry
- Enzymatic Synthesis
Background:
- Daidzein is valuable in medicine and health products but scarce naturally.
- Enzymatic hydrolysis is an efficient and eco-friendly method for daidzein preparation.
- Deep eutectic solvents (DESs) offer a novel, green reaction medium for biotransformations.
Purpose of the Study:
- To develop and optimize an enzymatic method for daidzein production from daidzin using DESs.
- To screen and identify the most effective DES co-solvent for enhancing bioconversion efficiency.
- To evaluate the reusability and overall efficiency of the DES medium in daidzein synthesis.
Main Methods:
- Screening of DESs based on enzyme activity, stability, and substrate solubility.
- Optimization of reaction parameters using response surface methodology (RSM).
- Enzymatic hydrolysis of daidzin in optimized Choline chloride/Ethylene glycol (ChCl/EG) DES medium.
Main Results:
- ChCl/EG (2:1, 30 vol %) was identified as the optimal DES co-solvent.
- Optimized conditions yielded a maximum daidzein conversion of 97.53% with >70% purity.
- The DES medium was reusable for six batches, maintaining >50% conversion, outperforming buffer systems.
Conclusions:
- The developed DES-based enzymatic hydrolysis is a mild, eco-friendly, and highly efficient method for daidzein production.
- This approach offers a simple, cost-effective process without harmful reagents.
- The reusability of DESs highlights their potential for sustainable biomanufacturing.
