Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prevalence and Impact of Social Risk in Patients With Inflammatory Bowel Diseases: National Estimates From the United States.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2025

Same author

AGA Clinical Practice Guideline on the Role of Biomarkers for the Management of Crohn's Disease.

Gastroenterology·2023

Same author

AGA Clinical Practice Guideline on the Role of Biomarkers for the Management of Ulcerative Colitis.

Gastroenterology·2023

Same author

Burden, Causes, and Outcomes of Hospitalization in Patients With Giant Cell Arteritis: A US National Cohort Study.

Arthritis care & research·2022

Same author

Impact of recurrent hospitalization for <i>Clostridioides difficile</i> on longitudinal outcomes in patients with inflammatory bowel diseases: a nationally representative cohort.

Therapeutic advances in gastroenterology·2022

Same author

Simplified Machine Learning Models Can Accurately Identify High-Need High-Cost Patients With Inflammatory Bowel Disease.

Clinical and translational gastroenterology·2022

Search research articles

Related Experiment Video

Updated: Mar 8, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

An Aptamer-based Sensor for Unchelated Gadolinium(III).

Osafanmwen Edogun¹, Tracy Y Chan¹, Nghia H Nguyen¹

¹Department of Chemistry and Biochemistry, California State University, East Bay.

Journal of Visualized Experiments : Jove

|January 25, 2017

Summary

A new aptamer-based sensor detects toxic unchelated trivalent gadolinium ions (Gd³⁺) in solution. This critical detection method offers nanomolar sensitivity for gadolinium-based contrast agent analysis.

More Related Videos

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

Related Experiment Videos

Last Updated: Mar 8, 2026

An Aptamer-based Sensor for Unchelated GadoliniumIII

An Aptamer-based Sensor for Unchelated GadoliniumIII

Published on: January 9, 2017

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

Area of Science:

Analytical Chemistry
Biomedical Engineering
Materials Science

Background:

Trivalent gadolinium ions (Gd³⁺) can be toxic and are present in gadolinium-based contrast agents due to incomplete synthesis or dissociation.
Accurate detection of free Gd³⁺ is crucial for patient safety and quality control of contrast agents.

Purpose of the Study:

To develop and validate an aptamer-based sensor for the selective and sensitive detection of unchelated Gd³⁺ in aqueous solutions.
To establish a reliable method for monitoring Gd³⁺ levels in samples relevant to medical applications.

Main Methods:

Design and synthesis of a specific aptamer sequence targeting Gd³⁺.
Development of a fluorescence-based assay utilizing the aptamer-sensor in a 384-well microplate format.
Characterization of sensor performance, including limit of detection and selectivity against other metal ions.

Main Results:

The aptamer-based sensor (Gd-sensor) exhibits a fluorescence response to increasing concentrations of Gd³⁺.
The sensor achieves a limit of detection in the nanomolar range (~100 nM) with a signal-to-noise ratio of 3.
The assay functions effectively in aqueous buffers at physiological pH and shows minimal reactivity towards common physiological ions, though cross-reactivity with other trivalent lanthanides was observed.

Conclusions:

The developed Gd-sensor provides a sensitive and selective method for detecting toxic unchelated Gd³⁺ in aqueous environments.
This assay is suitable for analyzing gadolinium-based contrast agents and assessing potential risks associated with free Gd³⁺.
The aptamer-based approach offers a valuable tool for quality control and safety assessment in biomedical applications involving gadolinium.