Automated countercurrent chromatography method development and process scale-up at GlaxoSmithKline

The choice of an appropriate combination of stationary phase and mobile phase is essential in any purification technique. To expedite this task, we use automated method screening, typically with high performance liquid chromatography (HPLC), to reduce the time to develop a scalable purification process. We have extended this approach to countercurrent chromatography (CCC) and describe a custom-configured system using automation to execute the solvent screening process. We also present results from three examples of method transfer from analytical to preparative scale using CCC systems from two different manufacturers.