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A novel monoterpenoid indole alkaloid with anticancer activity from Melodinus khasianus
Jie Zhou1, Jin-Hong Feng2, Lei Fang3
1School of Biological Science and Technology, University of Jinan, Jinan 250022, PR China; Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan 250014, PR China.
Abstract:
A novel monoterpenoid indole alkaloid with unprecedented 6/5/6/4/6 fused rings, khasuanine A (1), was isolated from the roots of Melodinus khasianus. The structure was determined by extensive analysis of its HR-MS, 1D-, and 2D-NMR spectra. Khasuanine A markedly inhibited the proliferation of PC3 cell with IC50 value of 0.45μM. Further study showed that khasuanine A was able to induce the apoptosis of PC3 cells by activation of caspase 3 and p53, and by inhibition of Bcl-2.
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