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Related Concept Videos

Lysosomes01:31

Lysosomes

26.8K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
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Lysosomal Hydrolases01:22

Lysosomal Hydrolases

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
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Autophagic Cell Death01:18

Autophagic Cell Death

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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
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Autophagy01:27

Autophagy

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
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Maturation of Endosomes01:28

Maturation of Endosomes

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The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
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Related Experiment Video

Updated: Mar 8, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Lysosomes get into the action.

Nancy R Gough1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|January 26, 2017
PubMed
Summary
This summary is machine-generated.

Activity-dependent lysosomal fusion with the plasma membrane drives dendrite remodeling. This process is crucial for neuronal plasticity and brain function.

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Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
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Area of Science:

  • Neuroscience
  • Cell Biology

Background:

  • Dendrite structure is vital for neuronal function and plasticity.
  • Lysosomes play key roles in cellular waste disposal and nutrient recycling.
  • Mechanisms regulating dendrite remodeling are not fully understood.

Purpose of the Study:

  • To investigate the role of lysosomal fusion with the plasma membrane in activity-dependent dendrite remodeling.

Main Methods:

  • Utilized advanced live-cell imaging techniques.
  • Employed genetic manipulation to track lysosomal dynamics.
  • Assessed changes in dendritic morphology in response to neuronal activity.

Main Results:

  • Demonstrated that neuronal activity triggers lysosomal fusion with the plasma membrane.
  • Showed that this fusion event directly contributes to the retraction and reshaping of dendrites.
  • Identified specific molecular players involved in the fusion process.

Conclusions:

  • Activity-dependent lysosomal exocytosis is a novel mechanism for dendrite remodeling.
  • This process is essential for adaptive changes in neuronal architecture.
  • Highlights lysosomes as key regulators of synaptic plasticity.