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Related Experiment Video

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Advanced Imaging Techniques for Investigation of Acellular Dermal Matrix Biointegration.

Brent R DeGeorge1,2, Bo Ning1,2, Lisa S Salopek1,2

  • 1Charlottesville, Va.

Plastic and Reconstructive Surgery
|January 26, 2017
PubMed
Summary

Advanced imaging techniques reveal real-time biointegration of acellular dermal matrices, detailing inflammation, cell infiltration, and new blood vessel growth. This provides a dynamic understanding of matrix-host interactions for reconstructive surgery.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Surgical Innovation

Background:

  • Biointegration of acellular dermal matrices (ADMs) involves inflammation, cellular infiltration, and angiogenesis.
  • Current evaluation relies on static histologic analysis at fixed time points.
  • Advanced imaging offers real-time, dynamic assessment of ADM biointegration.

Purpose of the Study:

  • To adapt and apply advanced imaging techniques for real-time serial assessment of ADM biointegration.
  • To investigate the host microenvironment in a murine model relevant to ADM-assisted breast reconstruction.
  • To provide a dynamic understanding of the biointegration process.

Main Methods:

  • Utilized two-photon microscopy and photoacoustic microscopy in a murine dorsal skin-fold window chamber model.
  • Employed dual-transgenic mice for fluorescently labeled cell tracking (perivascular, macrophage).
  • Noninvasively mapped microvasculature and oxygen consumption using photoacoustic microscopy, corroborated by histology.

Main Results:

  • Characterized robust inflammation at the ADM/host interface within the first 3 days.
  • Observed increased oxygen consumption and neoangiogenesis in the matrix border zone between days 10-14.
  • Documented vascular and inflammatory cell penetration into the ADM center after 21 days.

Conclusions:

  • The study provides a dynamic, real-time understanding of ADM biointegration.
  • Findings offer a template for differentiating various ADM products.
  • This knowledge can guide reconstructive surgeons in selecting optimal ADM adjuncts.