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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Nanoparticles Associate with Intrinsically Disordered RNA-Binding Proteins.

Alexander V Romashchenko, Tsung-Wai Kan1, Dmitry V Petrovski2

  • 1Department of Biochemistry, Erasmus Medical Center , Rotterdam 3015CE, The Netherlands.

ACS Nano
|January 26, 2017
PubMed
Summary
This summary is machine-generated.

Inorganic nanoparticles bind to intrinsically disordered proteins within cells. This interaction, driven by protein flexibility, may disrupt RNA processing and protein synthesis, potentially affecting cellular functions.

Keywords:
RNA-binding proteinsintrinsically disordered proteinsnanoparticlesproteomicsstress granules

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Area of Science:

  • Cell biology
  • Biochemistry
  • Nanotechnology

Background:

  • Nanoparticles can enter cells, but their interactions with the intracellular proteome are not well understood.
  • Understanding these interactions is crucial for predicting nanoparticle behavior and effects within biological systems.

Purpose of the Study:

  • To investigate the association between inorganic nanoparticles and intracellular proteins.
  • To identify the characteristics of proteins that bind to nanoparticles within a cellular context.

Main Methods:

  • Utilized nondenaturing in vitro nanoparticle pull-down assays with HeLa cytosolic protein extracts.
  • Analyzed the nanoparticle-associated proteome to identify specific protein types and characteristics.

Main Results:

  • Inorganic nanoparticles were found to associate with intrinsically disordered proteins (IDPs).
  • Protein side-chain flexibility, a characteristic of IDPs, was identified as a key factor in nanoparticle absorption.
  • The nanoparticle-associated proteome was significantly enriched in RNA-binding proteins and subunits of RNA-processing complexes.

Conclusions:

  • Nanoparticle-protein interactions are mediated by protein intrinsic disorder and flexibility.
  • These interactions suggest that nanoparticles may interfere with RNA processing and protein synthesis pathways within cells.
  • Potential disruption of these fundamental cellular processes could trigger a range of cellular responses to nanoparticle exposure.