Related Experiment Video
Updated: Mar 8, 2026
Depletion and Reconstitution of Macrophages in Mice
Published on: August 1, 2012
Octacosanol Attenuates Inflammation in Both RAW264.7 Macrophages and a Mouse Model of Colitis
Tianyi Guo1, Qinlu Lin1, Xinhua Li2
1Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
Abstract:
Octacosanol has multiple biological functions. In this study, the anti-inflammatory effect and molecular mechanism of octacosanol were evaluated by using dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. The colitis mouse model was induced by 3.0% DSS in 8-week ICR mice and octacosanol orally administered with 100 mg/kg/day. The results showed that octacosanol significantly improved the health status of mice and reduced DSS-induced pathological damage in the colonic tissues. Octacosanol obviously inhibited the mRNA and protein expression levels of pro-inflammatory factors of colonic tissues. In vitro, octacosanol administration significantly reduced the expression of mRNA or protein of pro-inflammatory cytokines and the phosphorylation of c-Jun N-terminal kinase and p38, and it also partly prevented LPS-induced translocations of NF-κB and AP-1. Octacosanol has anti-inflammatory effect, and its molecular mechanism may be involved in downregulating the expression of inflammatory factors and blocking of MAPK/NF-κB/AP-1 signaling pathway.
