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Related Experiment Video

Updated: Mar 8, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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Lung cells support osteosarcoma cell migration and survival.

Shibing Yu1, Mitchell Stephen Fourman1, Adel Mahjoub2

  • 1Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.

BMC Cancer
|January 27, 2017
PubMed
Summary

Lung cells promote osteosarcoma (OS) migration and survival. Disulfiram, an ALDH inhibitor, shows potential for treating metastatic OS by targeting these lung-mediated interactions.

Keywords:
Aldehyde dehydrogenaseDisulfiramLung microenvironmentMetastasisOsteosarcoma

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Area of Science:

  • Oncology
  • Cell Biology
  • Cancer Metastasis

Background:

  • Osteosarcoma (OS) is the most common primary bone tumor with poor survival rates for lung metastasis.
  • Current chemotherapy has not improved survival for metastatic OS.
  • Understanding the lung microenvironment's role in OS metastasis is crucial.

Purpose of the Study:

  • To explore lung cell and OS cell interactions in vitro.
  • To investigate how the lung microenvironment influences OS cell migration and survival.
  • To evaluate the efficacy of Disulfiram, an ALDH inhibitor, in the lung microenvironment.

Main Methods:

  • Co-culture of human OS and lung cell lines (SJSA-1, Saos-2, U-2 with HULEC-5a, MRC-5).
  • Use of lung cell-conditioned media to mimic the lung microenvironment.
  • Real-time cell analysis for migration/invasion, ALDH1 expression by RT-PCR, and ALP staining.

Main Results:

  • Lung cell-conditioned media significantly enhanced OS cell migration and induced morphologic changes.
  • SJSA-1 cells exhibited higher metastatic potential compared to other OS lines.
  • Increased alkaline phosphatase (ALP) staining observed in OS cells within the lung microenvironment.

Conclusions:

  • Lung endothelial cells (HULEC-5a) are attractants for OS cell migration, proliferation, and survival.
  • Aldehyde Dehydrogenase (ALDH) is implicated in lung-OS cell interactions.
  • Alkaline phosphatase (ALP) may serve as a biomarker for OS metastasis.