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Related Experiment Video

Updated: Jul 8, 2026

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
07:29

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Published on: May 25, 2011

Ankfy1 is dispensable for neural stem/precursor cell development.

Chao Weng1, Man Ding1, Lian-Sheng Chang2

  • 1Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China.

Neural Regeneration Research
|January 27, 2017
PubMed
Summary
This summary is machine-generated.

Ankyrin repeat and FYVE domain containing 1 (Ankfy1) protein is not essential for neural stem cell development. However, Ankfy1 knockout is lethal in early mouse embryos, indicating a critical role in embryonic development depending on genetic background.

Keywords:
Ankfy1embryofunctiongene knockoutgenetic backgroundnerve regenerationneural developmentneural regenerationneural stem/precursor cellsprotein

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09:19

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Area of Science:

  • Developmental biology
  • Genetics
  • Neuroscience

Background:

  • Ankyrin repeat and FYVE domain containing 1 (Ankfy1) is a membrane protein with limited study.
  • Ankfy1 exhibits specific expression in murine neural stem/precursor cells during early development.

Purpose of the Study:

  • To investigate the function of Ankfy1 in neural development.
  • To determine the impact of Ankfy1 gene knockout on murine development.

Main Methods:

  • Generation of Ankfy1 knockout mice on mixed and pure genetic backgrounds.
  • Immunofluorescence and in situ hybridization to assess neural development.
  • Polymerase chain reaction to confirm genotype and homozygote viability.

Main Results:

  • Ankfy1 null mice with a mixed genetic background showed no neural defects during development or in adulthood.
  • Ankfy1 knockout mice on a pure C57BL/6 genetic background exhibited embryonic lethality.
  • Homozygous Ankfy1 knockout was not attainable by embryonic day 11.5 in pure background mice.

Conclusions:

  • Ankfy1 protein is dispensable for neural stem/precursor cell function.
  • Ankfy1 plays a critical role in early embryonic murine development, with lethality being dependent on the genetic background.