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Updated: Mar 8, 2026

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Published on: September 28, 2018
Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation
Sara Martins1, Grégory Vert1, Yvon Jaillais2
1Institute for Integrative Biology of the Cell (I2BC), Centre National de la Recherche Scientifique, Commissariat à l'Energie Atomique, Université Paris Sud, Université Paris-Saclay, 91198, Gif-sur-Yvette, France.
Abstract:
Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions.
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