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Clathrin polymerization exhibits high mechano-geometric sensitivity.

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Cellular tension affects clathrin assembly and vesicle formation. Increased membrane tension reduces clathrin coat size, influencing cellular transport dynamics and protein polymerization.

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Area of Science:

  • Cellular biology
  • Biophysics

Background:

  • Cellular transport mechanisms are crucial for cell function.
  • The role of membrane tension in clathrin-mediated endocytosis is not fully understood.

Purpose of the Study:

  • To investigate how membrane tension influences clathrin assembly and vesicle formation.
  • To predict the energetic costs associated with membrane remodeling during vesicle growth under varying tension.

Main Methods:

  • Application of the Helfrich theory to model membrane mechanics.
  • Computational prediction of energetic costs for clathrin remodeling.
  • Experimental validation in mammalian cells.

Main Results:

  • Energetic cost of clathrin remodeling is sensitive to membrane tension and geometry.
  • A reduction in clathrin coat size is predicted and observed in intermediate tension regimes.
  • Membrane bending beyond the Ω shape significantly lowers the energy required for vesicle growth.

Conclusions:

  • Membrane tension and geometry are critical factors in clathrin-mediated vesicle formation.
  • Observed clathrin assembly modes (coated pits and plaques) may result from varying membrane tensions.
  • Mechano-geometric sensitivity is likely important for other membrane remodeling proteins.