Hymenobacter daeguensis sp. nov. isolated from river water

Area of Science:

• Microbiology
• Bacteriology
• Taxonomy

Background:

• The phylum Bacteroidetes harbors diverse bacterial species with significant ecological roles.
• The genus Hymenobacter, within the Cytophagaceae family, is known for its presence in various environments.
• Accurate taxonomic classification is crucial for understanding microbial diversity and function.

Purpose of the Study:

• To taxonomically characterize a novel bacterial isolate from the Han River, South Korea.
• To determine the phylogenetic and genotypic relationship of the isolate to known Hymenobacter species.
• To propose a new species designation based on comprehensive polyphasic analysis.

Main Methods:

• Isolation and cultivation of bacterial strain 16F3Y-2^T from the Han River.
• Polyphasic taxonomic characterization including 16S rRNA gene sequencing.
• Genomic DNA G+C content determination, respiratory quinone analysis, and fatty acid profiling.
• Phenotypic and chemotaxonomic analyses for differentiation.

Main Results:

• Strain 16F3Y-2^T is a Gram-negative, aerobic, non-motile, non-spore-forming rod.
• 16S rRNA gene sequence analysis placed the isolate within the genus Hymenobacter, closely related to Hymenobacter terrae.
• Chemotaxonomic data (MK-7, major fatty acids, polar lipids) and genomic G+C content (62.9 mol%) were determined.
• Low DNA-DNA relatedness values confirmed the isolate as a distinct genospecies, warranting new species status.

Conclusions:

• Strain 16F3Y-2^T represents a novel species within the genus Hymenobacter.
• The proposed name for this new species is Hymenobacter daeguensis sp. nov.
• The type strain is 16F3Y-2^T (=KCTC 52537^T =JCM 31654^T).