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Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness

Michiru Nishita1, Seung-Yeol Park2, Tadashi Nishio3

  • 1Division of Cell Physiology, Department of Physiology and Cell Biology, Kobe University, Graduate School of Medicine, Kobe, 650-0017, Japan. nishita@med.kobe-u.ac.jp.

Scientific Reports
|January 28, 2017
PubMed

Insights

Intraflagellar transport 20 (IFT20) is a new target of Ror2 signaling in tumors. IFT20 promotes tumor invasion by regulating Golgi structure and transport, offering new therapeutic targets.

Area of Science:

  • Cell Biology
  • Cancer Research
  • Molecular Biology

Background:

  • Ror2 receptor tyrosine kinase signaling drives invadopodia formation and tumor invasion.
  • The role of Ror2 signaling in tumors lacking primary cilia is not fully understood.

Purpose of the Study:

  • To identify novel targets of Ror2 signaling in tumor invasion.
  • To elucidate the mechanism by which Ror2 signaling promotes tumor invasiveness in non-ciliated tumors.

Main Methods:

  • Investigated the interaction between Ror2 signaling and intraflagellar transport 20 (IFT20).
  • Examined the regulation of Golgi-derived microtubules and Golgi ribbon formation by IFT20.
  • Assessed the impact of IFT20 on Golgi transport efficiency and tumor cell invasion.

Main Results:

  • IFT20 was identified as a novel target of Ror2 signaling in tumors lacking primary cilia.
  • IFT20 mediates Ror2-induced tumor invasiveness by regulating Golgi-derived microtubule nucleation via the GM130-AKAP450 complex.
  • IFT20 promotes Golgi ribbon formation, polarized secretion, and enhances Golgi transport efficiency.

Conclusions:

  • Ror2 signaling promotes tumor invasiveness through IFT20, which regulates Golgi structure and transport.
  • IFT20 represents a key mediator in Ror2-driven tumor cell migration and invasion.
  • These findings provide new insights into Ror2 signaling pathways and Golgi complex regulation in cancer.

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