Permanent atrial fibrillation impairs the function of circulating endothelial progenitor cells

to determine whether functions of endothelial progenitor cells (EPCs) were impaired in patients with permanent atrial fibrillation (AF).

35 patients with permanent AF (AF group) and 35 age and sex matched controls (control group) were collected. The numbers of circulating CD34⁺/KDR⁺ cells were determined with flow cytometry in the two groups. Cell proliferation, tube formation, nitric oxygen (NO) and vascular endothelial growth factor (VEGF) were assayed.

the numbers of CD34⁺/KDR⁺ cells were lower in the AF group than the control group (20.01 ± 12.66 /10⁵ vs 77.93 ± 58.93 /10⁵，p = 0.022). Colony formation unit (CFU) of EPCs were decreased in AF group compared to the control group（1.76 ± 0.59 CFU vs 3.45 ± 0.82 CFU, p = 0.0000). The AF group had lower cell proliferation ability than control group（0.401 ± 0.113 A vs 0.558 ± 0.130 A, p = 0.004). Tube formation ability was decreased in AF patients compared to controls（434.30 ± 96.22μm/mm² vs 568.09 ± 196.17μm/mm²，p = 0.041). AF patients had lower VEGF secretion than controls (27.35 ± 9.93 ng/L vs 41.86 ± 7.31 ng/L，p = 0.001），they also had lower NO secretion than controls（16.55 ± 6.92μmol/l vs 23.65 ± 5.48，p = 0.012).

proliferation, tube formation and paracrine of EPCs were reduced in patients with permanent AF.