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Actein inhibits glioma growth via a mitochondria-mediated pathway

Li-Qun Yuan, Yan-Ming Chen, Chao Sun

    Cancer Biomarkers : Section a of Disease Markers
    |January 28, 2017
    PubMed

    Insights

    Black cohosh triterpene glycoside Actein inhibits glioma cell growth and colony formation. Actein induces apoptosis via the mitochondria-mediated pathway, suggesting potential therapeutic use for glioma.

    Area of Science:

    • Pharmacology
    • Cancer Biology
    • Cell Biology

    Background:

    • Black cohosh (Actea racemosa) contains triterpene glycosides, including Actein.
    • Previous research indicates Actein inhibits human breast cancer cell growth.
    • Glioma is a primary brain tumor with limited treatment options.

    Purpose of the Study:

    • To investigate the anti-glioma effects of Actein.
    • To explore the underlying mechanisms of Actein's action on glioma cells.
    • To evaluate Actein's efficacy in a preclinical glioma model.

    Main Methods:

    • In vitro studies using U87 and U251 human glioma cell lines.
    • Assessment of cell viability, colony formation, and apoptosis markers (Bax, Bcl-2, cleaved caspase-3, -9, PARP1).
    • In vivo xenograft mouse model to assess tumor growth inhibition.

    Main Results:

    • Actein significantly inhibited glioma cell viability and colony formation in a dose- and time-dependent manner.
    • Actein induced apoptosis in glioma cells by modulating pro-apoptotic (Bax, cleaved caspase-3, -9, PARP1) and anti-apoptotic (Bcl-2) factors.
    • Actein suppressed tumor growth and induced apoptosis in a glioma xenograft model.

    Conclusions:

    • Actein exhibits significant growth inhibitory effects on glioma cells.
    • Mitochondria-mediated apoptosis is a key mechanism underlying Actein's anti-glioma activity.
    • Actein demonstrates potential as a therapeutic agent for glioma treatment.
    Keywords:
    Acteinapoptosisgliomagrowthmitochondria

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