Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expression of RUNX3 in skin cancers.

Clinical and experimental dermatology·2011

Same author

Malignant melanoma of the vulva: an extension of cutaneous melanoma?

Gynecologic oncology·2011

Same author

Molecular inflammation as an underlying mechanism of the aging process and age-related diseases.

Journal of dental research·2011

Same author

Comparative efficacy of commercial Mycoplasma hyopneumoniae and porcine circovirus 2 (PCV2) vaccines in pigs experimentally infected with M. hyopneumoniae and PCV2.

Vaccine·2011

Same author

Target structure induced suppression of the ionization cross section for very low energy antiproton-hydrogen collisions.

Physical review letters·2011

Same author

Synthesis of cold antihydrogen in a cusp trap.

Physical review letters·2011

Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026

Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026

Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026

Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026

Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026

Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 8, 2026

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Published on: March 15, 2012

Infectious disease models in zebrafish.

C Sullivan¹, M A Matty², D Jurczyszak¹

¹University of Maine, Orono, ME, United States.

Methods in Cell Biology

|January 29, 2017

Summary

This summary is machine-generated.

Zebrafish larvae offer a unique model for studying innate immune responses to pathogens due to their lack of adaptive immunity. Researchers describe methods for analyzing host-pathogen interactions in zebrafish, aiding infection and inflammation research.

Keywords:

Chemical screen Ecotoxicant Host-pathogen Immunity Infection Influenza Mycobacteria Pseudomonas Tuberculosis Zebrafish

More Related Videos

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Published on: January 20, 2017

Related Experiment Videos

Last Updated: Mar 8, 2026

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Published on: March 15, 2012

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Published on: October 8, 2018

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Published on: January 20, 2017

Area of Science:

* Infectious disease research
* Immunology
* Developmental biology

Background:

* Zebrafish (Danio rerio) are increasingly used as an alternative to mammalian models for studying host-pathogen interactions.
* Larval zebrafish lack a functional adaptive immune response, relying solely on innate immunity for protection against infection and injury.
* This developmental stage allows for the study of innate immune mechanisms without adaptive immune interference.

Purpose of the Study:

* To present methods for investigating host-pathogen interactions in zebrafish.
* To enable the study of innate immune responses, inflammation, and cellular behaviors during infection.
* To highlight the utility of zebrafish for ecotoxicant and chemical screening studies.

Main Methods:

* Systemic infection models in zebrafish larvae.
* Localized infection models for detailed analysis of host cell responses.
* High-resolution visualization of host-microbe interactions facilitated by larval optical clarity.

Main Results:

* Established methods allow for the isolation and study of innate immune responses to pathogens.
* Demonstrated the feasibility of analyzing host cell migration and behavior during infection.
* Zebrafish models facilitate high-resolution visualization of host-pathogen dynamics.

Conclusions:

* Zebrafish provide a powerful and versatile model for studying host-pathogen interactions and innate immunity.
* The described methods are adaptable for various downstream applications, including ecotoxicology and chemical screens.
* Zebrafish offer advantages such as genetic tractability, conserved genomes, and optical transparency for infection research.