Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

744
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
744
Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

2.0K
Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
2.0K
Prodrugs01:30

Prodrugs

4.5K
Prodrugs are a class of pharmaceutical compounds that undergo a biotransformation process within the body to be converted into a pharmacologically active drug. Prodrugs are designed to improve the therapeutic properties of the parent drug, such as enhancing bioavailability, increasing stability, or reducing toxicity. The concept of prodrugs revolves around modifying the chemical structure of the original drug to make it more effective or convenient for administration.
Prodrugs help overcome...
4.5K
Protein-protein Interfaces02:04

Protein-protein Interfaces

14.9K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.9K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

258
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
258
Drug Biotransformation: Overview01:16

Drug Biotransformation: Overview

4.0K
Pharmaceutical substances known as xenobiotics are predominantly lipophilic and nonionized. This enables them to permeate lipid bilayers, such as cell membranes, and interact with intracellular target receptors. Lipophilic drugs have an advantage in crossing biological barriers and reaching their intended sites of action. However, lipophilic drugs often have a restricted capacity for renal expulsion or elimination from the body. When these drugs enter the kidneys and undergo glomerular...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effective spectrum-based antibiotic resistance index for monitoring resistance in Gram-negative bacilli.

Antimicrobial stewardship & healthcare epidemiology : ASHE·2026
Same author

Packed red blood cell transfusion in the very low birth weight infant: Aggressive, conservative, or neither?

Seminars in fetal & neonatal medicine·2026
Same author

Optimal Spectrum Antibiotic Ratio for assessing antibiotic utilisation in sepsis caused by gram-negative bacilli.

EBioMedicine·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Overview of the Knowledge Management Center for Illuminating the Druggable Genome.

Drug discovery today·2024
Same author

Correction: Exploring DrugCentral: from molecular structures to clinical effects.

Journal of computer-aided molecular design·2023
Same journal

Evaluation of temporal preservation in synthetic longitudinal patient data.

Journal of biomedical informatics·2026
Same journal

ARKE: An ontology-driven framework for automated mapping of local radiology procedure terms to the LOINC-RadLex playbook using large language model.

Journal of biomedical informatics·2026
Same journal

A validation-driven training controller for cross-lingual biomedical NER via reinforcement learning-based adaptive loss weighting.

Journal of biomedical informatics·2026
Same journal

ASP-HR: An Adaptive Spatial Perception and Hierarchical Reasoning mechanism for document-level biomedical relation extraction.

Journal of biomedical informatics·2026
Same journal

Beyond Accuracy: Safety-Centered guidelines for the evaluation of LLM-based therapy recommendation systems for chronic multimorbidity patients.

Journal of biomedical informatics·2026
Same journal

DeepEN: A deep reinforcement learning framework for personalized enteral nutrition in critical care.

Journal of biomedical informatics·2026
See all related articles

Related Experiment Video

Updated: Mar 8, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.6K

Protein biomarker druggability profiling.

Subramani Mani1, Daniel Cannon1, Robin Ohls1

  • 1University of New Mexico Health Sciences Center, Albuquerque, NM 87131, United States.

Journal of Biomedical Informatics
|January 30, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a novel computational approach for identifying potential drug targets by analyzing protein biomarkers in neonatal sepsis. The method aids in evaluating and proposing new drug targets for diseases.

Keywords:
Druggability profilingMachine learningMechanistic annotationNeonatal sepsisPathway analysisProtein biomarkers

More Related Videos

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K
Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

3.8K

Related Experiment Videos

Last Updated: Mar 8, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.6K
Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets
06:40

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

1.8K
Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

3.8K

Area of Science:

  • Biochemistry
  • Computational Biology
  • Pharmacology

Background:

  • Biomarker discovery is crucial for understanding disease mechanisms and identifying therapeutic targets.
  • Current methods for drug target evaluation can be time-consuming and lack integration with pathway information.

Purpose of the Study:

  • To develop and demonstrate an automated, interactive method for drug target evaluation using ranked biomarkers.
  • To apply this approach to identify potential drug targets in neonatal sepsis.

Main Methods:

  • Incorporated mechanistic and causal annotations of ranked biomarkers into a contextual framework of disease-linked biochemical pathways.
  • Utilized a focused proteomic profile of sera from 127 infants (39 with late-onset neonatal sepsis, 88 controls).
  • Applied an interactive druggability profiling algorithm (DPA).

Main Results:

  • Successfully demonstrated the potential of the developed computational approach.
  • Identified ranked protein biomarkers associated with neonatal sepsis.
  • Facilitated drug-target evaluation and proposed new drug targets within disease pathways.

Conclusions:

  • The developed automated and interactive method offers a promising strategy for drug discovery and development.
  • This approach can accelerate the identification of novel drug targets by integrating biomarker data with pathway analysis.
  • The findings highlight the utility of DPA in analyzing complex biological data for therapeutic applications.