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Oat Anther Culture and Use of DH-Lines for Genetic Mapping
Elina Kiviharju1, Sirpa Moisander2, Pirjo Tanhuanpää2
1Natural Resources Institute Finland, Green Technology, Myllytie 1, Jokioinen, 31600, Finland. elina.kiviharju@luke.fi.
Abstract:
Possibility to make doubled haploids (DHs) from varying crossing populations is a useful tool for enhancing cultivar breeding, and a source of valuable material for genetic research. Oats is reported to be recalcitrant in anther culture with low response and genotype dependency. However, the best recoveries reported have reached up to 30 green regenerants per 100 isolated anthers, which clearly addresses the potential of this technique. In this chapter, one successful oat anther culture protocol is described in detail. Due to the total homozygosity reached in one generation, DH-lines are also an excellent material for genetic mapping. In this chapter, the use of DH-mapping population for marker analyses and linkage mapping is presented.
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