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Single-Cell Metabolomics.

Samy Emara1, Sara Amer1,2, Ahmed Ali1,2

  • 1Faculty of Pharmacy, Misr International University, Cairo, Egypt.

Advances in Experimental Medicine and Biology
|January 30, 2017
PubMed
Summary

Single-cell metabolomics is advancing, overcoming challenges in analyzing cellular variations. Live single-cell mass spectrometry (LCSMS) enables precise profiling for medicine and agriculture.

Keywords:
Circulating tumor cellDrug discoveryLive single-cell mass spectrometryMass spectrometryMatrix-assisted laser desorption ionizationMetabolomicsNanodevicesSingle cell

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Area of Science:

  • Cellular biology
  • Biochemistry
  • Analytical chemistry

Background:

  • Cellular dynamics involve complex, non-synchronous processes like movement, division, and communication.
  • Single-cell metabolomics is crucial for understanding phenotypic variations but faces challenges.
  • Low sample volumes, analyte concentrations, and gentle sampling techniques hinder accurate profiling.

Purpose of the Study:

  • To highlight the progress and challenges in single-cell metabolomics.
  • To discuss the role of advanced technologies and analytical techniques.
  • To emphasize the growing importance of live single-cell mass spectrometry (LCSMS).

Main Methods:

  • Review of advanced technologies like nanodevices and microfluidic arrays.
  • Discussion of analytical techniques such as matrix-assisted laser desorption ionization (MALDI).
  • Focus on live single-cell mass spectrometry (LCSMS) for sample quality and precision.

Main Results:

  • Despite barriers, advanced technologies are improving metabolomics profiling.
  • MALDI offers high-throughput methodology for cellular analysis.
  • LCSMS is transitioning from theoretical to practical applications.

Conclusions:

  • The field of metabolomics is progressing towards single-cell level analysis.
  • LCSMS is a key technology enabling precise and valuable discoveries.
  • Applications span medicine, pharmaceuticals, and agricultural industries.