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TGF-β1 activation in human hamstring cells through growth factor binding peptides on polycaprolactone surfaces.

J Crispim1, H A M Fernandes2, S C Fu3

  • 1Department of Developmental Bioengineering, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Drienerlolaan 5, 7500 AE Enschede, The Netherlands; Bioinspired Molecular Engineering Laboratory of the MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Hallenweg 15, 7522 NB Enschede, The Netherlands.

Acta Biomaterialia
|January 31, 2017
PubMed
Summary

Biomaterials functionalized with growth factor-binding peptides capture endogenous growth factors, enhancing tendon and ligament healing. This approach avoids exogenous growth factor administration, accelerating tissue repair.

Keywords:
Growth factor binding peptideInflammationTGF-β1Tendon/LigamentVascularization

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Soluble growth factors (GFs) enhance tendon and ligament (T/L) healing but require complex, expensive, and regulated administration at supraphysiological concentrations.
  • Spatial immobilization of GFs is critical for effective T/L healing.
  • Current methods for GF delivery are often suboptimal, necessitating novel strategies.

Purpose of the Study:

  • To develop and evaluate biomaterials functionalized with GF-binding peptides for capturing endogenous GFs.
  • To investigate if these functionalized biomaterials can promote T/L healing by presenting captured GFs in a spatially controlled manner.
  • To overcome the limitations of exogenous GF administration for T/L repair.

Main Methods:

  • Modification of polycaprolactone (PCL) films with transforming growth factor β1 (TGF-β1)-binding peptides.
  • Assessment of GF capture and biological activity using reporter cell lines and immunocytochemistry.
  • In vitro evaluation of collagen matrix production in human primary tendon cells.
  • In vivo implantation in rats to assess GF accumulation, inflammatory response, fibrogenesis, and vascularization.

Main Results:

  • PCL films functionalized with TGF-β1-binding peptides successfully captured and presented biologically active TGF-β1.
  • Immobilized TGF-β1 significantly increased collagen matrix production in tendon cells.
  • In vivo studies showed enhanced endogenous TGF-β1 accumulation on functionalized films.
  • Functionalized films promoted inflammatory cell recruitment, fibrogenic response, and vascularization around the implant.

Conclusions:

  • Biomaterials functionalized with GF-binding peptides offer a novel strategy for capturing endogenous GFs, promoting T/L healing.
  • This approach overcomes the need for exogenous supraphysiological GF administration, simplifying and potentially reducing the cost of T/L repair.
  • Functionalized biomaterials can impart novel biological properties to medical devices, accelerating T/L healing.